Vitamin D has emerging scientific support for its role in maintaining vaginal health, particularly in postmenopausal women. The vaginal epithelium contains vitamin D receptors, and studies suggest that vitamin D may help maintain the integrity of vaginal tissue, support epithelial cell proliferation, and contribute to mucosal immune defense. Clinical trials have investigated the effect of vitamin D supplementation and local application on symptoms of vaginal atrophy, dryness, and overall vaginal health. Several small randomized controlled trials have shown that vitamin D supplementation or vaginal suppositories can improve vaginal cell maturation and alleviate symptoms of atrophic vaginitis, especially in women with low estrogen levels. However, not all studies have demonstrated consistent benefits, and the quality of evidence is still considered moderate. The role of vitamin D in premenopausal women’s vaginal health is less well established. Current clinical guidelines do not universally recommend vitamin D specifically for vaginal health, but there is growing interest in its potential as an adjunct to standard therapies. Overall, while more large-scale, well-designed studies are needed, there is a scientific rationale and some evidence supporting the use of vitamin D to support the vaginal body system, particularly for postmenopausal women experiencing symptoms of atrophy or dryness.