Premna species, particularly Premna integrifolia (also known as Agnimantha), have a long history of use in traditional medicine systems such as Ayurveda for the treatment of various infectious conditions, including those believed to be caused by bacteria. Classical Ayurvedic texts mention Premna as an ingredient in multi-herb formulations used for managing fever and infections. Traditionally, the root and leaves are prepared as decoctions or pastes and are believed to exhibit antimicrobial and anti-inflammatory properties. From a scientific perspective, research into the antibacterial activity of Premna is limited but growing. Some in vitro studies have demonstrated that extracts of Premna species possess mild to moderate inhibitory effects against certain bacterial strains, including Staphylococcus aureus and Escherichia coli. Phytochemical analyses indicate the presence of flavonoids, alkaloids, and terpenoids, which may contribute to these effects. However, most studies are preliminary, do not use standardized extracts, and lack rigorous clinical trials in humans. In summary, while Premna is traditionally used to support the treatment of bacterial infections, scientific validation remains limited and is not robust enough to fully substantiate its efficacy. The evidence level is therefore rated as 2, reflecting traditional use with some supporting but insufficient scientific studies.