Missouri Snakeroot (Polygala senega, also called Seneca snakeroot) has been traditionally used by Native American groups and early settlers to address a range of ailments, including those involving the lymphatic system such as swollen glands. Historical herbal texts and ethnobotanical records note its application as a remedy for "swollen glands," sore throat, and as a general alterative (a substance believed to gradually restore health and proper function to the body, particularly the lymphatic and glandular systems). The roots were often used in decoctions or poultices applied to swollen or inflamed areas.

However, there is minimal scientific research specifically validating the efficacy of Missouri Snakeroot for treating swollen lymph glands. The evidence underlying its use is primarily anecdotal and based on traditional herbal medicine practices rather than clinical studies. Modern herbal references may mention its historical use for lymphatic support, but do not offer robust scientific backing. Most pharmacological investigations of Polygala senega have focused on its expectorant and anti-inflammatory properties, with little direct connection to lymphatic or glandular health.

In summary, Missouri Snakeroot's use for swollen glands is rooted in tradition, with a low level of evidence (1 out of 5), and there is no substantial modern scientific validation for this purpose.